By Ralf Kühn, Wolfgang Wurst, Benedikt Wefers

ISBN-10: 1493929313

ISBN-13: 9781493929313

​This quantity presents a accomplished selection of protocols on new expertise throughout quite a few version organisms.  Chapters describe species-specific the right way to generate new mutants the content material is done by way of chapters on common TAL effectors, TAL aspect DNA binding ideas, TALEN aim web site prediction, and techniques for the effective building of TALEN coding areas. whereas addition bankruptcy concentrate on the appliance of TALEN as sequence-specific nucleases and TAL dependent gene activators or inhibitors and the visualization of chromatin dynamics in stay cells.  Written within the hugely profitable Methods in Molecular Biology series layout, chapters comprise introductions to their respective issues, lists of the required fabrics and reagents, step by step, without difficulty reproducible laboratory protocols, and tips about troubleshooting and fending off recognized pitfalls.

Authoritative and state of the art, TALENs: tools and Protocols aims to make sure profitable leads to the additional learn of this very important field.

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Maeder ML, Linder SJ, Reyon D, Angstman JF, Fu Y, Sander JD et al (2013) Robust, synergistic regulation of human gene expression using TALE activators. Nat Methods 10:243–246 32. Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F (2012) Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun 3:968 33. Streubel J, Blücher C, Landgraf A, Boch J (2012) TAL effector RVD specificities and efficiencies. Nat Biotechnol 30:593–595 34. Yang J, Zhang Y, Yuan P, Zhou Y, Cai C, Ren Q et al (2014) Complete decoding of TAL effectors for DNA recognition.

Lackner G, Moebius N, Partida-Martinez L, Hertweck C (2011) Complete genome sequence of Burkholderia rhizoxinica, an Endosymbiont of Rhizopus microsporus. J Bacteriol 193:783–784 65. Partida-Martinez LP, Hertweck C (2005) Pathogenic fungus harbours endosymbiotic bacteria for toxin production. Nature 437: 884–888 66. de Lange O, Wolf C, Dietze J, Elsaesser J, Morbitzer R, Lahaye T (2014) Programmable DNA-binding proteins from Burkholderia provide a fresh perspective on the TALE-like repeat domain.

Nat Biotechnol 31: 833–838 Osborn MJ, Starker CG, McElroy AN, Webber BR, Riddle MJ, Xia L et al (2013) TALENbased gene correction for epidermolysis bullosa. Mol Ther 21:1151–1159 Richter A, Streubel J, Blücher C, Szurek B, Reschke M, Grau J et al (2014) A TAL effector repeat architecture for frame shift binding. Nat Commun 5:3447 Bonas U, Conrads-Strauch J, Balbo I (1993) Resistance in tomato to Xanthomonas campestris pv. vesicatoria is determined by alleles of the pepper-specific avirulence gene avrBs3.

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TALENs: Methods and Protocols by Ralf Kühn, Wolfgang Wurst, Benedikt Wefers

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