By Nobuhiro Takahashi
This is often one of many first books to target the dynamic element of proteomes. The booklet introduces proteomics to the newcomer, experiences the theoretical points of proteomics and its state-of-the paintings applied sciences, in addition to a few organic purposes utilizing "classical" proteomic know-how. The ebook additionally provides a brand new notion, the Dynamome, or the expression of a entire molecular set that participates within the entire dynamic strategy of a chain of mobile events.Content:
Chapter 1 evaluation of Proteomics (pages 1–61):
Chapter 2 Proteomic instruments for research of mobile Dynamics (pages 63–166):
Chapter three Dynamics of practical mobile equipment: From Statics to Dynamics in Proteomic Biology (pages 167–247):
Read or Download Proteomic Biology Using LC-MS: Large Scale Analysis of Cellular Dynamics and Function PDF
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Additional info for Proteomic Biology Using LC-MS: Large Scale Analysis of Cellular Dynamics and Function
Among the key potentials of this strategy is the capability not only to screen for previously unknown gene products but also to assign them, along with other known but poorly characterized gene products, to particular subcellular structures. This strategy is called subcellular proteomics (75) or organellar proteomics (74). Subcellular structures targeted by this strategy include not only entire organelles (such as the nucleus and mitochondria) but also nonorganelle structures (such as the postsynaptic density and raft), which can be isolated by traditional subcellular fractionation typically using sucrose density-gradient ultracentrifugation, and comprise a focused set of proteins that fulfill discrete but varied cellular functions.
Immobilized aminoethylcysteine can be used to capture proteins/peptides with Ser/Thr phosphorylation sites. An immobilized library of partially degenerate phosphopeptides biased toward a particular protein kinase phosphorylation motif is used to isolate phospho-binding domains that bind to proteins phosphorylated by specific kinase. Top–down MS/bottom–up MS/MS. In vivo labeling with 12C and 13C tyrosine and immunoisolation with anti-phosphotyrosine antibody for quantitative comparison. 32 P labeling and Edman sequencing/2DE.
1-3-2 Activity-Based Protein Profiling/ Enzyme Substrate Proteomics Activity-based profiling (ABP) provides a strategy for identifying proteins associated with a particular biological activity—typically enzymatic activity (57)—and utilizes synthetic chemistry to create tools and assays for the characterization of protein samples of high complexity (64). The ABP strategy simplifies a complex biological mixture of proteins before analysis by labeling a specific set of related proteins with an affinity or fluorescence tag (Fig.
Proteomic Biology Using LC-MS: Large Scale Analysis of Cellular Dynamics and Function by Nobuhiro Takahashi