By K.V.R. Chary

ISBN-10: 1402066791

ISBN-13: 9781402066795

ISBN-10: 1402066805

ISBN-13: 9781402066801

During educating NMR to scholars and researchers, we felt the necessity for a text-book which could disguise smooth tendencies within the software of NMR to organic platforms. This e-book caters to the wishes of: (i) graduate scholars who regularly study such concepts from senior post-docs within the laboratory; (ii) those who find themselves no longer specialists in NMR yet desire to comprehend if a selected challenge in animal, plant, clinical and pharmaceutical sciences may be spoke back by way of NMR; and (iii) people who find themselves specialists in chemistry and biochemistry and need to understand how NMR gives you them info on structural or practical element of proteins, nucleic acids, cells and tissues, human and plant organs and different organic fabrics. This e-book builds a way of data move among the rookies and the specialists in NMR as utilized to all elements of existence sciences.

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Extra info for NMR in Biological Systems: From Molecules to Humans

Example text

The chemical shifts of nuclear spins can change due to a direct delocalization of the electron spins at various nuclear sites (presence of finite spin densities), referred as contact term, or due to the anisotropy of the g tensor of the electron spin (pseudo-contact term). The nuclear spins in BASIC CONCEPTS IN NMR SPECTROSCOPY 23 paramagnetic systems relax mainly through electron-nuclear interactions. Endogenous paramagnetic reagents in the medium such as metal ions can influence the relaxation of spins present in diamagnetic molecules.

9). Secondly, for nuclei with spin quantum number > ½ the nuclear charge distribution is not spherically symmetric. These nuclei therefore have an additional property called the quadrupole moment (Q). When the electric field gradient at the site of the nucleus produced by electronic clouds is asymmetric, such an interaction manifests directly in the NMR spectrum. For an electronic cloud having an axial symmetry, the magnitude of such an interaction is given by (eq)(eQ) or e2qQ to the first order, where e is the electronic charge and q is the z-component of the electric field gradient at the nuclear site.

Magnets with narrow bores (typically a few cm), are used mostly for high-resolution solution work. The sample used in a high-resolution NMR experiment is generally around 600 Pl and is taken typically in a sample tube of 5 mm in diameter. Wider bore magnets are used for studies on solid-state materials, which require additional hardware within the magnet bore such as the magic-angle spinning assembly and equipment for work over a wide temperature range. When working with humans and animals, lower magnetic field strengths are used because of safety considerations.

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NMR in Biological Systems: From Molecules to Humans by K.V.R. Chary

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