By Matthew N. Davies, David E. Gloriam, Darren R. Flower (auth.), Craig W. Stevens (eds.)
The objective of the characterization and discovery of G protein-coupled receptors, arguably crucial type of signaling molecules in people and different vertebrates, has spawned various very important methodologies. In Methods for the invention and Characterization of G Protein-Coupled Receptors, specialists within the box current the very most up-to-date at the equipment and expertise used to represent and detect novel mechanisms of GPCRs which, in lots of situations, can be utilized on to layout experiments for the reader’s specific GPCR of curiosity and their particular street of research. Divided into 4 handy sections, this particular quantity covers GPCRs within the genome, trafficking of GPCRs, GPCRs at the membrane, in addition to the rules of those key receptors. Chapters additionally function an enormous part referred to as “Future instructions” which supplies the reader an perception into advances quickly to be learned in each one sector. Written for the preferred Neuromethods sequence, this e-book comprises the type of specified description and implementation recommendation that's an important for purchasing optimum results.
Authoritative and state of the art, Methods for the invention and Characterization of G Protein-Coupled Receptors serves as an incredible consultant for scientists decided to extra our wisdom of crucially very important set of receptors.
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Additional resources for Methods for the Discovery and Characterization of G Protein-Coupled Receptors
Splicing of the intron downstream. Mol Cell Biol 17:4667–4676. Chan RC, Black DL (1997) Conserved intron elements repress splicing of a neuron-specific c-src exon in vitro. Mol Cell Biol 17:2970. Chan RC, Black DL (1995) Conserved intron elements repress splicing of a neuron-specific c-src exon in vitro. Mol Cell Biol 15:6377–6385. Xu R, Teng J, Cooper TA (1993) The cardiac troponin T alternative exon contains a novel purine-rich positive splicing element. Mol Cell Biol 13:3660–3674. Ryan KJ, Cooper TA (1996) Muscle-specific splicing enhancers regulate inclusion of the cardiac troponin T alternative exon in embryonic skeletal muscle.
Each gel element contains a specific oligonucleotide probe chemically bound to polyacrylamide. The 3D structure of the PAA gel-based elements allows immobilization of much greater amounts of the oligonucleotide probe within each element compared to 2D arrays, therefore increasing the intensity of the signal from an element. Hybridization and enzymatic reactions within the acrylamide gel more closely resemble liquid-phase reactions, rather than solid-phase reactions on 2D support. , determining the thermodynamic parameters of the nucleic acid duplexes (21) and de novo sequencing of short nucleic acids (22).
2 Alternative Pre-mRNA Splicing of G Protein-Coupled Receptors 21 splicing of GPCRs. Alternative splicing can lead to generating multiple protein products or isoforms from single gene. These protein products can encode truncated or novel proteins by losing segments of the protein or gaining new protein sequences, or changing the amino acid sequence through a reading-frame shifting or an early stop codon. Alternative splicing of GPCRs often leads to truncations at the N-terminus, C-terminus and transmembrane segments, or generation of new N-terminal and C-terminal sequences.
Methods for the Discovery and Characterization of G Protein-Coupled Receptors by Matthew N. Davies, David E. Gloriam, Darren R. Flower (auth.), Craig W. Stevens (eds.)