By Michiel Mueller

ISBN-10: 0819460435

ISBN-13: 9780819460431

This ebook offers a finished account of the idea of picture formation in a confocal fluorescence microscope in addition to a realistic instruction to the operation of the tool, its barriers, and the translation of confocal microscopy facts. The appendices supply a short connection with optical idea, microscopy-related formulation and definitions, and Fourier theory.

Contents

- Symbols and abbreviations
- Preface
- Confocal fluorescence microscopy
- Implementation
- sensible limits
- Digitization
- Miscellaneous topics
- Appendix A: parts of optical theory
- Appendix B: formulation, family and definitions
- Appendix C: Fourier theory
- Bibliography
- Index

Show description

Read Online or Download Introduction to Confocal Fluorescence Microscopy, Second Edition PDF

Best instruments & measurement books

Download PDF by Robert Buchheim: Sky is Your Laboratory: Advanced Astronomy Projects for

For the skilled novice astronomer who's pondering if there's something invaluable, beneficial, and everlasting that may be performed together with his or her observational talents, the answer's, "Yes, you could! " this is often the e-book for the skilled novice astronomer who's able to take a brand new step in his or her astronomical trip.

Get Mental Health Practice in a Digital World: A Clinicians PDF

The aim of the psychological overall healthiness perform in a electronic global: A Clinicians advisor publication is to organize clinicians to appreciate, significantly overview, and include well-designed and demonstrated applied sciences that experience the potential for reworking the entry, affordability, and responsibility of psychological healthcare.

Download e-book for kindle: Remote Observatories for Amateur Astronomers: Using by Gerald R. Hubbell

Beginner astronomers who are looking to improve their functions to give a contribution to technology want glance no farther than this consultant to utilizing distant observatories. The members disguise how one can construct your personal distant observatory in addition to the prevailing infrastructure of industrial networks of distant observatories which are to be had to the beginner.

Download e-book for kindle: Utilization Management in the Clinical Laboratory and Other by Kent Lewandrowski, Patrick M. Sluss

This ebook is the 1st accomplished textual content on usage administration within the medical laboratory and different ancillary prone. It presents a close assessment on how one can determine a winning usage administration software, targeting such matters as management, governance, informatics, and alertness of usage administration instruments.

Extra info for Introduction to Confocal Fluorescence Microscopy, Second Edition

Example text

To illustrate this point, consider the results of a numerical simulation shown in Fig. 13. 13 The influence of Poisson noise between two distributions, shifted spatially such that the maximum of the one coincides with the first minimum of the other (Rayleigh criterion). In this example, on average (a) 10, (b) 100, and (c) 1000 photons are detected from each intensity maximum. The reproducibility of each measurement is illustrated by the three subsequent measurements shown horizontally for each case.

For instance, consider the detection of fluorescence emitted from a sample. 12 The Rayleigh criterion. Two components of equal intensity are resolved when the maximum of one coincides with the first minimum of the other. This results in an effective drop of the intensity between the two maxima of ∼20%. Confocal Fluorescence Microscopy 15 domly distributed over time. To determine the average fluorescence rate, the total number of photons emitted over a given time interval can be measured. Because of the random arrival of photons at the detector, repeating the measurement will yield a distribution of values.

17 mm. For some air- and water-immersion objectives, a special correction collar permits correction of spherical aberration for various cover-glass thicknesses. Note that additional spherical aberration is introduced when the refractive index of the object does not match that of the immersion medium (see Sec. 4). 4 Microscope objective types Microscope manufacturers have introduced various types of microscope objectives that compensate for various orders of optical aberration. In addition to spherical aberration, chromatic aberration and field curvature are the most important aberrations.

Download PDF sample

Introduction to Confocal Fluorescence Microscopy, Second Edition by Michiel Mueller


by John
4.3

Rated 4.67 of 5 – based on 7 votes