By M. J. Gasson, G. F. Fitzgerald (auth.), Michael J. Gasson, Willem M. De Vos (eds.)
A leading reference quantity for geneticists, foodstuff technologists and biotechnologists within the educational and commercial sectors. Fermentations with lactic acid micro organism make certain very important traits similar to flavor, shelf-life, and nutrition values. New equipment of nutrients creation require quickly and trustworthy manufacture, which has ended in a dramatic surge of curiosity within the genetic, microbiological and biochemical houses of lactic acid bacteria.
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Additional resources for Genetics and Biotechnology of Lactic Acid Bacteria
These observations strongly suggest that the nisin transposon transposes by this latter mechanism and that one of the directly repeated 5'-TTTTTG-3' sequences is present on and physically introduced with the element. The large 70 kb size of the transposon reflects the presence of a complex operon for the biosynthesis of nisin, as well as the genes necessary for sucrose fermentation, conjugation and transposition. It has also been shown that nisin transposons encode genes for a bacteriophage insensitivity phenomenon (Gonzalez and Kunka, 1985) and for production of the enzyme N5-(carboxyethyl) ornithine synthase (Donkershoot and Thompson, 1990).
A:O) Summary of the properties of the IS elements described in the lactic acid bacteria. , I:l:l 0 Z '"> (") ::l trl Z trl Cl 00 GENE TRANSFER SYSTEMS AND TRANSPOSITION 29 riophage resistance gene abi4l6 isolated from L. lac tis subsp. lactis IL4l6. , 1991). During analysis of nisin genes a new IS element, IS904 was found to be located near to one end of the conjugative transposon Tn530l that also encoded genes for nisin biosynthesis and sucrose fermentation. , 1990). A closely related insertion sequence, ISlO76 has been characterized by Huang et al.
Power et al. (1988) obtained a 300- to 1000-fold increase when L. lactis subsp. lactis LM0230 was incubated with 2 kU/ml of lysozyme for 20 minutes at 37°C in electroporation buffer. 5 M sucrose. Kim et al. (1992) also treated Pediococcus acidilactici cells with lysozyme prior to electroporation but in this case, transformants were plated on media to which sucrose was not added. Practically all electroporation protocols describe the chilling of cells before the application of the electric field pulse followed by incubation of the treated cells on ice for a short period before dilution and plating on selective media.
Genetics and Biotechnology of Lactic Acid Bacteria by M. J. Gasson, G. F. Fitzgerald (auth.), Michael J. Gasson, Willem M. De Vos (eds.)