By Muhammad Sohail

ISBN-10: 0849321417

ISBN-13: 9780849321412

content material: Gene silencing by means of RNA interference and the function of small interfering RNAs --
fundamentals of siRNA layout and chemical synthesis --
Oligonucleotide scanning arrays within the layout of small interfering RNAs --
siRNA construction by means of in vitro transcription --
construction of siRNAs with the appliance of deoxyribozymes --
creation of siRNA in vitro by means of enzymatic digestion of double-stranded RNA --
Plasmid-mediated intracellular expression of siRNAs --
Lentiviral vector-mediated supply of si/shRNA --
Exogenous siRNA supply: protocols for optimizing supply to cells --
RNAi in drosophila mobile cultures --
RNAi in caenorhabditis elegans --
supply of RNAi reagents in C. elegans via microinjection --
RNAi in drosophila embryos --
RNAi in xenopus laevis oocytes --
RNAi in xenopus embryos --
An RNAi-based genomic library for ahead genetics within the African trypanosome --
RNAi within the malaria parasite plasmodium --
Silencing the expression of cysteine proteases (falcipains) in plasmodium falciparum utilizing RNA interference ways --
RNAi in transgenic animal types --
RNA interference in mouse versions --
Virus-induced RNA silencing in crops --
RNA silencing in vegetation: biolistic supply of RNAi reagents --
Agro-infiltration: a flexible software for RNAi stories in plants.

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Extra resources for Gene silencing by RNA interference : technology and application

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Nuclear Factor YY1 inhibits Transforming Growth Factor {beta}- and Bone Morphogenetic Protein-induced cell differentiation, Mol. Cell. , 23, 4494, 2003. 43. Li, T. , Identification of the gene for vitamin K epoxide reductase, Nature, 427, 541, 2004. 44. , Using RNA interference to identify genes required for RNA interference, Proc. Natl. Acad. Sci. , 99, 4191, 2002. pmd 35 7/26/2004, 10:57 AM 3 Oligonucleotide Scanning Arrays in the Design of Small Interfering RNAs Valentine M. 1 INTRODUCTION Small interfering RNAs are powerful gene silencing tools.

2 Rational siRNA Design: Potency and Longevity One attribute of rationally designed siRNAs is that on average, the potency and longevity of these duplexes is greater than that of conventionally designed molecules. 6). 5 Silencing data comparing siRNAs designed by random or by an advanced rational selection method developed by the scientists at Dharmacon, Inc. The gene targets are human SEAP (NM_001632) and firefly Luciferase (U47298). Randomly selected siRNAs (light gray bars) are designated by their relative position in the coding sequence.

Li, T. , Identification of the gene for vitamin K epoxide reductase, Nature, 427, 541, 2004. 44. , Using RNA interference to identify genes required for RNA interference, Proc. Natl. Acad. Sci. , 99, 4191, 2002. pmd 35 7/26/2004, 10:57 AM 3 Oligonucleotide Scanning Arrays in the Design of Small Interfering RNAs Valentine M. 1 INTRODUCTION Small interfering RNAs are powerful gene silencing tools. 3–5 As this approach became more widely used, it was apparent that only about 50% of siRNAs were effective, and the determinants of activity were unclear.

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Gene silencing by RNA interference : technology and application by Muhammad Sohail


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