By M. A. Hayat

ISBN-10: 0123339200

ISBN-13: 9780123339201

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Extra resources for Fixation for Electron Microscopy

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Studies of mouse pancreas embedded in water-miscible methac- 48 2. Factors Affecting t h e Quality of Fixation rylates at low temparatures indicate that Tris and phosphate buffers are less efficient in preserving cellular integrity than is the bicarbonate buffer (Cope, 1968). The buffer is compatible with uranyl salts. PM Buffer The PM buffer is a nonionic buffer used initially for in vitro polymerization of microtubules (Weisenberg, 1972) and stabilizes microtubules when used during fixation with glutaraldehyde.

5. It should not be used with aldehydes, since it reacts with these fixatives and the reaction product has no buffering value in the physiologically important range of pH. Veronal acetate cannot be stored in the absence of 47 3uffers the fixative because it easily becomes contaminated by bacteria and mold. It has the advantage of not precepitating uranyl acetate. Membranes appear to be preserved better when O s 0 4 is buffered with veronal than with other buffers. 5 and is a biological inhibitor.

Phosphate buffers decrease nuclear mass more than that caused by veronal buffer. Phosphate buffers extract nonchromosomal proteins in the nucleus, whereas veronal buffer removes part of the chromatin proteins. There is some evidence indicating that phosphate buffers may cause swelling of intracellular organelles, whereas cacodylate does not seem to exert this effect. Phosphate buffers may precipitate polyvalent cations and lead and uranium salts, which may result in general artifactual precipitation.

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Fixation for Electron Microscopy by M. A. Hayat


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