By Anant Pai
Used to be Chanakya a demon or a man?. His competitors have been confounded. the coed grew to become flesh presser used to be continuously a step sooner than his enemies. He led his king Chandragupta to greatness and set his nation at the street to peace and prosperity. Then, for generations of destiny rulers, he compiled an invaluable advisor - the famously canny Arthashastra.
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The application of this methodology gives fractions with distinct polarities, easing the analyzes of the chemical structure, functional groups and CHCI3 fraction Chromatography silica gel eluted solvenb (35-70 Mesh) 1- CH:2 CI2 Filtered over a silica gel (70-230 Mesh) 2- MeOH 1-CH 2 C1 2 2- EtOAc 3- MeOH Fracbon 1 Chromatography silica gel (70-230 Mesh) eluted solvents: hexane- Ct-QC12- MeOH (9:1'0 - 0'0 1) increasmg polarity obtained fracbons 40 FractIOns eluted With hexane- Ct-QC12 (73 - 5:5) Chromatograpy silica gel (70-230 Mesh) hexane- Chromatography silica gel (70·230 Mesh) CH:2CI:2- MeOH (1:1·0:1) (64 - 0:1) CH2CI2- MeOH (1 0 - 0 1) Fractions eluted with hexane- CIi1CI2 (6'4 - 0,1) Fractions eluted With CH2CI2- MeOH (91,01) Chromatography silica gel (70-230 Mesh) hexane- C~12 (8'2 - 0:1) CH~I2" MeOH (1:0 - C~12 FractIOn 2 and Fraction 3 0 1) Chromatography silica gel (70-230 Mesh) hexane- C~12 (6:4 - 0'1) CH:2CI2" MeOH (1:0 - 0'1) 'Column Chromatograpy HPLC TLC Crystallization Scheme 1.
Fatty acids, from seed oils, and hydrocarbons, from diesel oil. Column open chromatography using silica impregnated with potassium hydroxide is another chromatographic method used to analyze copaiba oils. This modified silica works not only as a normal phase but as a cation exchange process too. This silica enables the elution of all the metabolites that are not carboxylic acids, which could be eluted with using methanol and mobile phase. Since copaiba oils have large amounts of diterpenic carboxilic acids, this kind of pre-fractionation is very useful and helps the isolation of the two classes of metabolites: sesquiterpenes and diterpenes.
P. and Hanazaki, N. (2006), As pesquisas etnodirigidas na descoberta de novos farmacos de interesse medico e farmaceutico: fragilidades e perspectivas. Revista Brasileira de Farmacognosia, 16(Supl): 678-689. C. (1982). Estudos silviculturais de uma populac;iio natural de Copaifera multijuga Hayne-Leguminosae, na Amazonia Central. Acta Amaz6nica, 12: 75-89. J. L. (1995). A diterpene from Mikania obtusata active on Trypanosoma cruzi. Planta Medica, 61: 85-86. F. M. (2005). Ion-exchange chromatography applied to the isolation of acidic fraction from copaiba (Copalfera multijuga) oil and from sacaca (Croton cajucara).
Chanakya (ACK-Visionary) by Anant Pai