By Golder Wilson

ISBN-10: 0071375783

ISBN-13: 9780071375788

ISBN-10: 0071389377

ISBN-13: 9780071389372

Offers clinical scholars 500 questions, solutions, and factors to organize for the biochemistry and genetics sections of the USMLE Step 1. the recent version comprises many new questions within the two-step medical structure to simulate the USMLE Step 1.

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Extra resources for Biochemistry & Genetics: PreTest Self-Assessment & Review

Sample text

The answer is b. (Murray, pp 412–434. Scriver, pp 3–45. Sack, pp 3–29. ) The Ames test is a rapid and relatively inexpensive bacterial assay for determining mutagenicity of potential toxic chemicals. Since many chemical carcinogens are mutagenic, it seems obvious that damage to DNA is a central event in carcinogenesis as well as mutagenesis. Dr. qxd 11/14/01 10:36 AM Page 27 DNA Structure, Replication, and Repair Answers 27 ified not to grow in the absence of histidine because of a mutation in one of the genes for the biosynthesis of histidine.

Murray, pp 412–434. Scriver, pp 3–45. Sack, pp 3–29. ) Sufficient DNA for analysis can be obtained by amplification of leukocyte DNA using the polymerase chain reaction (PCR). qxd 24 11/14/01 10:36 AM Page 24 Biochemistry and Genetics Short segments of DNA (oligonucleotide primers) are designed to be complementary to areas flanking the DNA region of interest—in this case, the portion of the β-globin gene that may harbor the sickle cell anemia (141900) mutation. Some 20 to 30 cycles of cooling (to anneal the primers), synthesis (with heat-stable DNA polymerase), and heating (to melt the DNA and allow the next cycle) can amplify the targeted DNA segment over 1 million-fold.

A. RNA primer is synthesized b. DNA polymerase III synthesizes DNA c. Helicase (rep protein) continuously unwinds duplex DNA at the replication fork during synthesis d. Nucleoside monophosphates are added in a 5′ to 3′ direction along the growing DNA chain e. qxd 11/14/01 10:36 AM Page 17 DNA Structure, Replication, and Repair 17 23. Which of the following statements describing restriction endonucleases is true? a. b. c. d. e. They always yield overhanging single-stranded ends They recognize methylated DNA sequences They recognize triplet repeats They cleave both strands in duplex DNA They always yield blunt ends 24.

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Biochemistry & Genetics: PreTest Self-Assessment & Review by Golder Wilson


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