By A. T. Ganesan

ISBN-10: 0122741552

ISBN-13: 9780122741555

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187. , Carroll, L. and Sueoka, N. (1980). Genetics 9 8 , 809. Karamata,D. D. (1970). Gen. Genet. 108, 227. , Tanaka, T. and Sueoka, N. (1983). Proc. Natl. Acad. Sei. USA 80, 574. Matsumura, M . , Imanaka, T. and Alba, S. (1984). J . Bacteriol. 160, 413. Ogasawara, N . , Moriya, S. and Yoshikawa, H. (1985). Nucleic Acids Res. 13, 2267. Pabo, C O . T. (1984). Ann. Rev. Biochem. 53, 293. Scheer-Abramowitz, J . J. and Dubnau, D. (1980). Plasmid 6, 67. Sueoka, N. and Hammers, J . (1974). Proc. Natl.

To test the importance of preventing double homologous cross-over, we chose to construct both plasmids which would fit with the notion of the perfect couple in host DN1280 and plasmids which harboured segments flanking both sides of the dal-1280 deletion. Two representative plasmid constructions are shown in Figure 6. pDN1290 makes a perfect couple with DN1280 since it lacks the EcoR5 site whereas pDN1130 closely resemble pDN1290, but harbours both the EcoR5 site and a ca. 400 bp flanking segment for which reason it does not make a perfect couple with DN1280.

Natl. Acad. Sei. USA 71, 4787. Sueoka, N . , Tanaka, T. and Winston, S. (1980). IJH Genetics and Biotechnology of Bacilli (A. Ganesan and J . Hoch, eds), p. 79. Tanaka, T. and Sueoka, N. (1983). J . Bacteriol. 154, 1184. White, K. and Sueoka, N. (1973). Genetics 73, 185. Winston, S. and Sueoka, N. (1980). Proc. Natl. Acad. Sei. USA 77, 2834. IN THE BACILLUS SUBTILIS MUTANT DNA 3 7 1 Simone J. Seror-Laurent Gilles Henekes Alain Levine Françoise Vannier Institut de Microbiologie Universite Paris XI Orsay, France I.

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