By N. Chiu, et. al.
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Additional info for Advances in Immunoassay Technology
1999). Intrabody construction and expression. II. A synthetic catalytic Fv fragment. J Mol Biol, 291(5), 1129-1134. Orcutt, K. , Slusarczyk, A. , Bhushan, K. , Frangioni, J. , & Wittrup, K. D. (2011). Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging. Nucl Med Biol, 38(2), 223-233. , & Ohlin, M. (2006). A focused antibody library for improved hapten recognition. J Mol Biol, 357(2), 607-620. , & Bradbury, A.
These have atleast one separation step allowing the differentiation of reacted from un-reacted materials. The enzymatic activity is quantified either in bound state or free fraction by an enzyme catalyzed process of a relatively nonchromatic substrate to highly chromatic product. ,1976]. In ELISA, antibody immobilized on the solid support detects the specific antigen present in the sample and this immune complex is detected by a high turn-over enzyme conjugated antibody. The excess of reagents are washed off in each step and the subsequent substrate interaction yields a coloured product either for the direct visualization or for measuring the optical density.
Stemmer, W. , & Minshull, J. (1999). DNA shuffling of subgenomic sequences of subtilisin. Nat Biotechnol, 17(9), 893-896. Ohage, E. , & Steipe, B. (1999). Intrabody construction and expression. II. A synthetic catalytic Fv fragment. J Mol Biol, 291(5), 1129-1134. Orcutt, K. , Slusarczyk, A. , Bhushan, K. , Frangioni, J. , & Wittrup, K. D. (2011). Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging. Nucl Med Biol, 38(2), 223-233.
Advances in Immunoassay Technology by N. Chiu, et. al.